Combined effects of soft drinks and nicotine on <i>Streptococcus mutans</i> metabolic activity and biofilm formation

The purpose of this study was to explore the effects nicotine on activity Streptococcus mutans (S. mutans) in soft drinks. Regular drinks contain large proportions high-fructose corn syrup (HFCS), which increases S. resulting high-caries risk compared with sugar-free Nicotine use exhibits a strong correlation increased biofilm formation. chosen were (Coca-Cola Classic, Diet Coke, Coca-Cola Zero Sugar, Caffeine-Free Coca-Cola, Sugar). grown overnight tryptic soy broth; diluted broth supplemented 1.0% sucrose followed by dilution 1:3. Total growth absorbance and determined spectrophotometry, measured determine formation, metabolic quantified. One-way ANOVA showed considerable effect for HFCS caffeine presence their interaction all measures. Results caffeinated colas demonstrated significant inhibiting formation nicotine. Nicotine-induced In conclusion, smokers should consider versions minimize chance developing dental caries dut reduction